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Figure 7. Effect of RPA and gp32 on the helicase activity of FFA-1. (A) Helicase reactions containing the indicated amounts of FFA-1 and RPA (in nM). (B) Helicase reactions containing the indicated amounts of FFA-1 (in nM) and gp32 (in μM). In both A and B, the second lanes from the right contain buffer only, whereas the rightmost lanes contain the heated DNA substrate. All the reactions also contain 2.5 μM (in nucleotides) DNA and 2 mM ATP. Indicated on the left of each gel are the migration positions of double-stranded DNA markers. Image published in: Chen CY et al. (2001) © 2001 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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