Xenbase Image ID: 119673

Figure 8. Effect of GST–FFA-1 fusion proteins on FFA-1 helicase activity. (A) In the presence of RPA. Lanes 1–10 contain 2 nM FFA-1 and 60 nM RPA, but lanes 1–9 also contain the various fusion proteins at the indicated final concentrations (in μM). Lane 11 contains the substrate incubated in buffer only and lane 12 contains the heated substrate. (B) In the absence of RPA. Lanes 1–3 contain 5 nM FFA-1 and GST-Xho (0.75 μM), GST-Stu/Xho (0.75 μM), and GST-Stu (1.5 μM). Lane 4 contains 5 nM FFA-1 only, lane 5 contains the substrate incubated in buffer only, and lane 6 contains the heated substrate. More FFA-1 was used in this experiment to better detect the weak helicase activity in the absence of RPA. The film was also exposed four times as long as that of A. All the reactions contain 2.5 μM DNA and 2 mM ATP. Image published in: Chen CY et al. (2001) © 2001 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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