Xenbase Image ID: 122530

Figure 2. Immunolocalization of CVAK104.A) HeLa cells were either treated with siRNA against CVAK104 or untreated (control), then mixed and labelled with anti-CVAK104 antibody. Asterisks indicate CVAK104-depleted cells. Scale bar: 10 μm. B–D) Double labelling for CVAK104 and coat proteins. B) HeLa cells were double labelled for CVAK104 and CHC. The two patterns show significant colocalization. C) HeLa cells were double labelled for CVAK104 and AP-1 γ. Again the two patterns show significant colocalization. D) HeLa cells were double labelled for CVAK104 and AP-2 α. The two patterns show no significant colocalization. Scale bars: 5 μm. E) HeLa cells were treated with BFA (20 μg/mL) for 5 minutes at 37 °C or treated with medium only (control), and labelled with anti-CVAK104 antibody. Upon BFA treatment, CVAK104 loses its membrane association and becomes largely cytosolic. Scale bars: 10 μm. CHC, clathrin heavy chain. Arrowheads indicate areas of colocalisation. Image published in: Borner GH et al. (2007) Image downloaded from an Open Access article in PubMed Central. © 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd Larger Image Printer Friendly View

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