Xenbase Image ID: 122532

Figure 4. CVAK104 kd results in specific depletion of syntaxin 8 and vti1b from CCVs.Cell homogenates and CCV fractions were prepared from control and siRNA-treated cells and analysed by Western blot. CVAK kd1 and CVAK kd2 denote samples from two independent kd experiments. Approximately 10 μg protein of whole cell lysates or 2.5 μg of CCV fractions were loaded in each lane. Although the four SNAREs, syntaxin 7, syntaxin 8, vti1a, and vti1b, are all sorted by clathrin, only syntaxin 8 and vti1b are depleted from CCVs in a CVAK104 kd. CHC, clathrin heavy chain; kd, knockdown. Image published in: Borner GH et al. (2007) Image downloaded from an Open Access article in PubMed Central. © 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd Larger Image Printer Friendly View

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