XB-IMG-122645
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Figure 7. Switching sequence differences in AC region between mSlo1 and dSlo1 either singly or in combination does not switch Ca2+ sensitivity of BKCa gating. (A) Steady-state G-V relations of m[d-Motif2] and m[d-Motif3]. Dotted lines are G-V relations of mSlo1. Parameters of the Boltzmann fits (solid lines): for m[d-Motif2], at 0 [Ca2+]i, V1/2 = 195.7 mV, z = 1.1; at 100 μM [Ca2+]i, V1/2 = 8.2 mV, z = 1.2; for m[d-Motif3], at 0 [Ca2+]i, V1/2 = 213.2 mV, z = 1.1; at 100 μM [Ca2+]i, V1/2 = 9.2 mV, z = 1.2. (B) Steady-state G-V relations of m[d-Motif12] and m[d-Motif13]. Dotted lines are G-V relations of mSlo1. Parameters of the Boltzmann fits (solid lines): for m[d-Motif12], at 0 [Ca2+]i, V1/2 = 186.5 mV, z = 1.2; at 100 μM [Ca2+]i, V1/2 = 22.2 mV, z = 1.1; for m[d-Motif13], at 0 [Ca2+]i, V1/2 = 211.9 mV, z = 1.4; at 100 μM [Ca2+]i, V1/2 = 38.4 mV, z = 1.3. (C) Free energy of activation provided by Ca2+ binding for the chimeric channels. Free energy change for each channel was in response to [Ca2+]i change shown in parentheses for mSlo1 plotted alongside each group. Image published in: Krishnamoorthy G et al. (2005) Copyright © 2005, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |