Xenbase Image ID: 122921

Figure 4. CENP-E motor activity is essential for metaphase chromosome alignment. (A) Experimental scheme for Xenopus extract manipulation. (B) Immunoblot of CENP-E (340 kD) and Rod (220 kD; a loading control) in mock-depleted, CENP-E–depleted, wild-type CENP-E–supplemented, and rigor CENP-ET91N–supplemented Xenopus extracts. (C) Recombinant full-length CENP-E partially rescued chromosome alignment, whereas rigor CENP-E failed at rescue. Red, X-rhodamine tubulin; green, DAPI. (D) Quantification of structures formed in Xenopus extracts. More than 200 spindles were scored each in three independent depletion/add-back experiments. Error bars represent SD. Bar, 10 μm. Image published in: Kim Y et al. (2008) © 2008 Kim et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page