XB-IMG-123021
Xenbase Image ID: 123021
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FIGURE 3. A yeast Rtn1p mutant with defects in localization and oligomerization also cannot shape tubules in vivo. A, mutations in Rtn1 Mutant 1, Mutant 2, and Mutant 91 that affect the ability of the protein to localize exclusively to the tubular ER. The residues altered in Mutant 1 are shown in blue, and those in Mutant 2 are shown in green. K48I is responsible for the altered localization of Mutant 2. The combined mutations of Mutant 91 are indicated in purple. The two membrane-embedded regions are indicated in red. B–D, fluorescence intensities normalized to prebleach values plotted over time from FRAP analyses of Rtn1-GFP Mutant 1 (B), Mutant 2 (C), or Mutant 91 (D) expressed in wild-type S. cerevisiae cells. Error bars indicate ± S.E., n = 4–8 cells. E, the indicated GFP fusions were constitutively expressed under their endogenous promoters in rtn1Δrtn2Δyop1Δ cells. The ER was visualized by focusing on either the center or periphery of the cell. Note that wild-type, Mutant 1, and Mutant 2 Rtn1-GFP restore tubular ER structure, whereas Mutant 91 and the control protein Sec63-GFP do not. Image published in: Shibata Y et al. (2008) Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View |