Xenbase Image ID: 123025

FIGURE 7. The reticulons and DP1 form oligomers in higher eukaryotes. A, quantitated FRAP analyses of mammalian GFP-Rtn3c under ATP-depleted (red, n = 7) or non-depleted (blue, n = 14) conditions. B, mammalian Sec61β-GFP under ATP-depleted (red, n = 9) or non-depleted (blue, n = 6) conditions. FRAP data from ATP-depleted cells were compared with those from non-treated cells from Fig. 6. C, Xenopus membranes solubilized in either 1.25% digitonin or 2% Nonidet P-40 were fractionated by 10–30% w/v sucrose gradient centrifugation, analyzed by SDS-PAGE, and immunoblotted with antibody against Xenopus Rtn4. Molecular weight standards in the sucrose gradient are as indicated. D, isolated membranes of COS-7 cells expressing HA-DP1 or HA-Rtn3c were treated with increasing concentrations of EGS, analyzed on SDS-PAGE, and immunoblotted with anti-HA antibody. Asterisks, number of monomers cross-linked. Image published in: Shibata Y et al. (2008) Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

Return to previous page