Xenbase Image ID: 123026

FIGURE 8. Overexpression of a reticulon stabilizes the tubular ER in the absence of microtubules in mammalian cells. A, a typical COS-7 cell expressing GFP-Sec61β (green), where microtubules have been depolymerized by nocodazole treatment (immunostained with anti-αtubulin, red), has collapsed, sheet-like peripheral ER. The lower row of panels shows an enlargement of the boxed region from the top panels. B, a typical COS-7 cell expressing GFP-Rtn3c (green) after microtubule depolymerization (stained in red) leads to an accumulation of bright foci containing Rtn3c but also retains much more tubular ER. The lower row of panels shows an enlargement of the boxed region from the top panels. Scale bar, 10 μm. Image published in: Shibata Y et al. (2008) Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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