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Figure 2. Identification of xRPA as the stimulating factor for xDNA2. (A) Western blot of xRPA (p70 subunit) in the heparin flow through before and after incubation with ss-oligo beads. (B) The stimulating activity in heparin flow through, before and after incubation with oligo beads, and pure xRPA (at 90 nM and 30 nM). The reactions were terminated with 1% SDS, heated at 95°C for 5 min, and then analyzed on an 8% TAE/PAGE. (C) Nuclease assay with different DNA substrates. The substrates were 48-mers in either ss- or ds-form. They were attached to magnetic beads, leaving either the 5′ or the 3′-end (of the 32P-labeled strand) accessible to the nuclease. The assay condition was similar to that in (B). Image published in: Liao S et al. (2008) © 2008 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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