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Figure 4. Effect of xDNA2 on 5′ ss-tail degradation. Denatured pUC19 DNA (2 ng/µl) labeled at the 3′-end was incubated in xDNA2-depleted or mock-depleted NPE supplemented with buffer or the purified xDNA2 (to ∼5% of the level of the endogenous xDNA2). Time points were treated with SDS/proteinase K and separated on a 1% TAE agarose gel. The gel was first stained with SYBR Gold to detect total DNA and then dried for exposure to X-ray film to detect 32P. The band migrated just below the ss-pUC19 DNA is the RNA in NPE. Image published in: Liao S et al. (2008) © 2008 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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