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Figure 5. Effect of xDNA2 on end unwinding. (A) Principle of the unwinding assay. Thin line: normal nucleotides; thick line: thio nucleotides; *, 32P label. (B) The thio 5′ oligo duplex precoated onto Streptavidin magnetic beads was incubated in xDNA2-depleted or mock-depleted NPE. Samples were separated into bead and supernatant fractions, treated with SDS/proteinase K and analyzed on a 10% native TAE PAGE. B, beads; S, supernatant. The SDS/proteinase K treatment in this study was at room temperature for 2 h, a condition not harsh enough to disrupt the binding of biotin with Streptavidin (1). Image published in: Liao S et al. (2008) © 2008 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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