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Figure 6. Effect of xDNA2 on SSA. The substrate, pRW4′, which carried two direct repeats at the partially filled-in XhoI protruding ends, was incubated in mock-depleted or xDNA2-depleted NPE (supplemented with xDNA2 or buffer) at room temperature. DNA samples were taken at the indicated times, treated with SDS/EDTA/Proteinase K, separated by TAE/agarose gel electrophoresis and detected by SYBR Gold stain. The SSA products include the band indicated by the arrow and a subset of the bands indicated by the bracket. The asterisks indicate the NHEJ products. Image published in: Liao S et al. (2008) © 2008 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View |