XB-IMG-123438
Xenbase Image ID: 123438
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FIGURE 4. The interaction of yNap1 and DNA with histone tetramer. A,
normalized fluorescence change as a function of histone tetramer binding to
(yNap1m*)-Alexa-546 (closed circles), tail-less
(H3/H4)2 (open squares), and H3(H113A)/H4 (plotted as
tetramer) (open squares). Inset of A is the
stoichiometry of (H3/H4)2 to yNAP dimer. (H3/H4)2 was
titrated against 1×10–7 m
(yNap1m*)-Alexa-546 to result in a stoichiometry of 1 histone
tetramer to 1 yNap1 dimer. B, normalized fluorescence change as a
function of DNA binding to (H3/H4(E63C))2-Alexa-488
(10–10 m). Buffer conditions were 20–50
mm Tris, pH 7.5, 1 mm dithiothreitol, 0.1 mg/ml bovine
serum albumin, and 300 mm NaCl. Image published in: Andrews AJ et al. (2008) Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View |