Xenbase Image ID: 123486

Figure 3. Single-molecule photo-bleaching of GFP-Orai in intact oocytesa, Store depletion induces formation of Orai punctae in Xenopus oocytes. Images were obtained by TIRF microscopy of oocytes expressing GFP-Orai together with StimV5His and show 40 × 40 μm regions in the animal hemisphere before (left) and 2 hr after (right) bath application of 10 μM TG in zero-calcium Ringer's solution. b, Oocytes expressing GFP-Orai together with C-Stim showed strong Ca2+ influx, whereas this was absent with expression of GFP-Orai or C-Stim alone. Pairs of traces show cytosolic [Ca2+] as reported by normalized fluorescence pseudoratio changes of Fluo-4 (F) and whole-cell voltage-clamp measurements of Ca2+-activated Cl- current (I) in response to a hyperpolarizing step from 0 mV to -120 mV (top trace). c, Representative TIRFM image, acquired before photobleaching, showing fluorescent spots (circled) sparsely distributed in the membrane of an oocyte expressing GFP-Orai together with C-Stim. Inset shows a magnified view of a small region (white box), with circular regions of interest used to measure bleaching steps overlaid on the image. Image published in: Penna A et al. (2008) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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