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Figure 1. Hipk1 interacts with Dsh and Tcf3.(A) 35S-Met-labelled Dsh precipitates in a complex with the partial-length Hipk1 GST-tagged clone from the yeast-2-hybrid screen, but not with GST alone. (B) Immunoprecipitation (IP) of 35S-Met-labelled Dsh with Myc-tagged Hipk1 (left panel), and reciprocal 35S-Met-labelled Hipk1 IP with Myc-tagged Dsh (right panel), using in vitro translated proteins. (C) IP of Dsh with Hipk1 when both proteins are recombinantly expressed in X. laevis embryos. (D) Endogenous Tcf3 IP with recombinantly expressed Myc-(K/R) Hipk1 in ventral X. laevis embryos. An anti-Spectrin antibody was used as a negative control for the IP (top) and as a loading control for the input (bottom). Image published in: Louie SH et al. (2009) Louie et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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