Xenbase Image ID: 123746

Fig. 1. Expression vector pVP62K. (a) Linear map showing key features of the vector and location of the Bar-CAT toxic cassette and 3′ homology region (3′-hmr) for Flexi Vector cloning. (b) Nucleotide and encoded protein sequence in the linker region near to the SgfI cloning site. The TVMV protease site is ETVRFQS, where proteolysis occurs between the Q and S residues. The fusion protein may be cleaved in the expression host due to the presence of a low level of TVMV protease produced by constitutive expression from pVP62K. The TEV protease site is ENLYFQA, where proteolysis occurs between the Q and A residues. After purification of the His8-tagged protein, the His8 tag can be removed by treatment with TEV protease to release an N-terminal AIA-target Image published in: Frederick RO et al. (2007) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2007 Larger Image Printer Friendly View

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