Xenbase Image ID: 123748

Fig. 3. Caliper LC90 analysis of His8-tagged proteins purified by Maxwell 16. Lanes LA and LB are molecular weight markers. Lanes A1–B2 are structural genomics target proteins (protein bands marked with ovals) with molecular weight ∼50–75 kDa. They were expressed in factorial evolved auto-induction medium containing selenomethionine [33] as an N-terminal fusion with MBP from pVP56K, a vector that does not give in vivo proteolysis of the fusion protein. Lane B3 contains His8-MBP (protein band marked with oval), while lane B4 (1.1 mg/ml) contains His8-GFP expressed from pVP62 after in vivo cleavage from MBP. Lanes with a purified expressed fusion protein with yield than 100 μg/ml are marked with a star (also see Table 2 ) Image published in: Frederick RO et al. (2007) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2007 Larger Image Printer Friendly View

Return to previous page