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Fig. 4. Small-scale purification screening of human embryonic stem cell proteins. Human stem cell proteins were expressed in E. coli B834 by auto-induction, liberated by in vivo proteolysis, and purified by the Maxwell 16 purification system. Table 2 provides further information on these proteins. Lane 1, molecular weight markers. Lanes 2 and 3, total cell lysate and eluted sample from purification of CCNF. No purified protein was detected. Lanes 4 and 5, C10orf96 was obtained in detectable amounts, but not sufficient for scale-up, along with two higher molecular weight contaminants. Lanes 6 and 7, His8-Tcl-1 was expressed, proteolyzed, and successfully purified. Lanes 8 and 9, NPM2 was expressed and proteolyzed, but only a small amount of protein was purified. In addition, the purified protein appeared to be partially degraded. Lanes 10 and 11, His8-GFP Image published in: Frederick RO et al. (2007) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2007 Larger Image Printer Friendly View

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