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Fig. 6. 1H–15N HSQC NMR spectra of Maxwell-purified proteins Tcl-1 and GFP. (a) 750 MHz spectrum of His8-Tcl-1 obtained at 35°C (1.75 mg in 250 μl of 10 mM KHPO4, pH 7, containing 50 mM KCl). The total NMR time required to obtain this spectrum was 9.5 h. (b) 600 MHz spectrum of His8-GFP obtained at 35°C (5.6 mg in 250 μl of 10 mM KHPO4, pH 7, containing 50 mM KCl). The total NMR time required to obtain this spectrum was 1 h Image published in: Frederick RO et al. (2007) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2007 Larger Image Printer Friendly View

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