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Fig. 8. Schematic of a purification screening protocol. Steps from obtaining a sequence-verified target in auto-cleavage vector pVP62K to identification of purified proteins. The transformed expression host is grown in auto-induction medium. Cells from production trials are loaded into the Maxwell 16 instrument for automated purification, and purified proteins are detected by Caliper LC90 capillary electrophoresis. Successful purification of a protein from auto-cleavage expression with yield exceeding 50 μg/ml of culture medium indicates feasibility of scale-up efforts Image published in: Frederick RO et al. (2007) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2007 Larger Image Printer Friendly View

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