Xenbase Image ID: 123804

Figure 2. Testing the GTPase flux modela–d, Embryos were injected with GFP-rGBD to monitor active Rho along with WT Mgc (b), Mgc R384A (c), or Mgc ΔGAP (d) and imaged during cytokinesis. Frames taken from time-lapse movies are shown. A single blastomere is outlined in red in the first frame, and the Rho activity zone is marked with an arrow in the second frame. Scale bars, 40 μm. e, Average Rho zone width / longitudinal diameter for multiple cells. Mean ± SE. Control, n = 12 embryos; WT Mgc, n = 9; R384A, n = 10; ΔGAP, n = 12. *p < 0.05; **p < 0.01; ***p < 0.005. f–i, Gene replacement experiments where embryos were injected with Mgc MO.2 to knock down endogenous MgcRacGAP along with mRNAs for WT Mgc (g), Mgc R384A (h), or Mgc ΔGAP (i). The embryos were also injected with GFP-rGBD to monitor active Rho. A single blastomere is outlined in red in the first frame, and the Rho activity zone is marked with an arrow in the second frame. Scale bars, 40 μm. j, Effects of constitutively-active Rho (CA-Rho) on Rho zone dynamics. Frames taken from time-lapse movies showing Rho activity dynamics during cytokinesis in control cells and cells expressing CA-Rho are shown. Cytokinetic Rho zones (arrows) are broader and brighter in cells expressing CA-Rho and are much slower to furrow. Moreover, the amount of Rho activity outside the equtorial regions is much higher in those cells expressing CA-Rho than the controls. Note that these phenotypes closely resemble those obtained with the R384A GAP-DEAD mutant. Scale bars, 40 μm. Image published in: Miller AL and Bement WM (2009) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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