Xenbase Image ID: 123822

Figure 1. Rtt109-Vps75 acetylates residues within the H3 tail. (a) MS/MS spectrum of histone H3 peptide K(ac)STGGK(ac)APR, residues 9-17, conclusively confirms H3K9 and H3K14 are acetylated within the protein. The observed b and y fragment ions, resulting from collison-induced dissociation of the peptide backbone, are labeled on the peptide sequence and in spectrum with their ion type, position and mass/charge (m/z). (b,c) MATa, bar1 strains of the indicated genotypes were arrested in G1 phase with α factor mating pheromone and released into a synchronous cell cycle in pheromone-free media for the indicated times at 30 °C. Whole-cell extracts were analyzed by immunoblotting with the indicated antibodies. (d) Immunoblot signal from anti-H3K9ac from the mid-S phase 30-min time point in b or c were quantified by chemiluminescence on a LAS-300 (Fujifilm) and calculated relative to the total H3 signal. The average percent acetylation relative to the wild-type (WT) strain for three independent experiments is depicted. (e) Immunoblot signal from anti-H3K14ac. Samples were treated and analyzed as in d. (f) Immunoblot signal from anti-H3K23ac. Samples were treated and analyzed as in d for two independent experiments. Error bars in d and e indicate s.d., and error bars in f indicate average error. Image published in: Berndsen CE et al. (2008) Image downloaded from an Open Access article in PubMed Central. © 2008 Nature Publishing Group Larger Image Printer Friendly View

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