Xenbase Image ID: 123823

Figure 2. Rtt109 stimulates histone-deposition activity of Vps75. Histone H3 and H4 deposition by Vps75 in the presence or absence of Rtt109 was monitored by supercoiling assay. DNA was unwound before adding to the deposition reaction, and deposition activity was quantified by measuring the density as a percentage of that of the total DNA that was supercoiled in each lane, which corrects for possible variations in loading. Values shown are averages of three independent experiments ± s.d. Shown at the right are representative control assays of no Vps75 or Rtt109 control, Vps75 only or Rtt109-Vps75 from the same gel. The zero time point for each assay used the same stock of relaxed DNA and is shown in the ‘No chaperone’ control. Gel images were inverted for clarity. Image published in: Berndsen CE et al. (2008) Image downloaded from an Open Access article in PubMed Central. © 2008 Nature Publishing Group Larger Image Printer Friendly View

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