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Figure 3. Primer extension on an end-blocked oligonucelotide by recombinant PCNA, RFC and Pol δ. (A) Schematic structure of the end-blocked primer/template. A biotin (B) is attached at the 5′ end of the template and is bound to a streptavidin (SA)-conjugated magnetic bead. (B) Primer extension reactions were conducted with indicated protein factors and the normal primer/49-nt G template as described in ‘Materials and Methods’ section. Image published in: Fazlieva R et al. (2009) © 2009 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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