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Figure 4. Complementation of Xenopus egg extracts with recombinant Pol δ. Single-stranded circular DNA was annealed with non-radioactive ssDNA primer (Figure 2) and used for replication synthesis in the presence of 32P-dATP. (A) DNA replication in Pol δ- or mock-depleted NPE. Samples taken at the indicated times were treated with SDS/EDTA/Proteinase K and separated by 1% TAE/agarose gel electrophoresis. (B) Rescue of the replication defect by the recombinant Pol δ. Image published in: Fazlieva R et al. (2009) © 2009 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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