XB-IMG-123860
Xenbase Image ID: 123860
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Figure 7. Nucleotide determination of products extended from 3′-mispaired primers. (A) Representative data of pyrosequencing. The ratio of the corrected nucleotide and the mispaired nucleotide was obtained from two peaks in the pyrogram (boxed). In this case with the 49 nt A:G primer, A is the mispaired nucleotide, while C is the corrected nucleotide opposite G. (B) Summary of pyroseqencing data. Efficiency of synthesis of fully extended products over unextended primers (quantitated from Figure 6) is presented along with the ratio of proofread products (dark area) and mispaired products (gray area). Deletion products from the 57-nt G:G primer were indicated as white area. (C) Three mechanisms for extension of the 57-nt G:G primer. The 3′-mispaired nucleotide is indicated with an asterisk. Image published in: Fazlieva R et al. (2009) © 2009 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View |