Xenbase Image ID: 124225

FIGURE 1. Lys38 is essential for MCT1 activity. Xenopus oocytes were injected with water or cRNA for wild type MCT1 or the mutant shown, and after expression for 3 days the uptake of l-[14C]lactate was determined as describe under “Experimental Procedures.” Where indicated the oocytes were treated with 0.2 mm DIDS under conditions designed to reveal reversible or irreversible inhibition. The conditions were chosen that gave incomplete inhibition so that either increases or decreases in inhibitor affinity caused by subsequent mutations could be readily detected. The data are shown as the means ± S.E. of 30–180 separate oocytes. The insets show a Western blot of crude membrane fractions (equal protein loading) and sections of oocytes subject to immunofluorescence microscopy to confirm that the lack of lactate transport by the K38Q (inset B) or K38R (inset C) mutants is not due to reduced MCT1 expression compared with wild type MCT1 (inset A). Inset D is water-injected. Scale bar, 20 μm. Image published in: Wilson MC et al. (2009) © 2009 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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