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FIGURE 4. The effects of trypsin digestion of DIDS-treated rat erythrocyte ghosts on the cross-linked product of MCT1 with embigin. In panel A, rat erythrocytes were incubated in the absence or presence of 100 μm DIDS for 1 h at 37 °C prior to preparation of ghosts and, where required, treatment with trypsin as described under “Experimental Procedures.” The proteins were subjected to SDS-PAGE and Western blotting using the antibodies against the C terminus or 7/8 loop of MCT1 or an antibody raised against the cross-linked product of MCT1 with embigin (16). In B data are shown for samples that have been treated with N-glycanase F (DGaseF) as described under “Experimental Procedures.” The data shown are representative of two or three experiments that gave similar results. Image published in: Wilson MC et al. (2009) © 2009 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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