Xenbase Image ID: 124251

Figure 2. Xenopus laevis Piwi proteins with bound piRNAs are immunoprecipitated by Y12 and contain sDMAs(a) Protein immunoprecipitates from indicated X. laevis tissues; Xili and Xiwi were identified by mass spectrometry (Supplementary Table 3).(b) Immunoprecipitates from X. laevis oocytes were probed on Western blots with indicated antibodies. Band with asterisk is bovine IgG from tissue culture supernatant of anti-Mili hybridoma.(c) RNA-immunoprecipitations from X. laevis.(d) Periodate oxidation and β-elimination of X. laevis piRNAs isolated from Y12 immunoprecipitates.(e) Nucleotide composition of X. laevis piRNAs.(f) Northern blot for XL-piR-3(g) In situ hybridization for XL-piR-3 in X. laevis oocyte; bar = 100μm Image published in: Kirino Y et al. (2009) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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