Xenbase Image ID: 124252

Figure 3. Drosophila PRMT5 (csul, dart5) is required for arginine methylation of Aub, Piwi and Ago3 proteins in ovaries(a) Western blots from wild-type (WT) or csul (dPRMT5) mutant (−/−) ovary. Piwi or Aub immunoprecipitates from ovary lysates were probed on western blots with anti-Piwi and anti-Aub antibody(b); or SYM11 and ASYM24 (c).(d) Ago3 immunoprecipitates from WT or csul mutant (−/−) ovary lysates were probed on Western blots (WB) with indicated antibodies.(e) Sequences of wild-type (WT) and mutant (M) Aub, where the four arginines that are substrates for methylation were substituted with lysines.(f) Anti-Flag immunoprecipitates of S2 cells stably transfected with WT or M Flag-Aub were probed on western blots with indicated antibodies.(g) Crosslinking of synthetic, radiolabeled piRNA to immunopurified WT or M Flag-Aub.(h) RNA immunoprecipitation.(i) Periodate oxidation and β-elimination of Drosophila piRNAs isolated from Piwi immunoprecipitates from wt or csul (−/−) mutant ovaries. Image published in: Kirino Y et al. (2009) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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