Xenbase Image ID: 124316

Figure 6. Three views of two models of the CFTR pore based on Sav1866 and a 5 ns molecular dynamics simulation. Insets show the portion of the protein that is enlarged in each panel, and dashed lines indicate the approximate boundaries of the lipid bilayer. Also, the contains two movies that offer an overall view of the two models. Cytoplasmic domains have been removed from the enlarged views. (A) Top view looking “down” into the pore from the extracellular side. (B) Side view with TMs 9, 10, and 12 partially removed to reveal the “pore-lining” face of TM6. (C) Side view from behind the TM3−4 loop. Residues indicated in black are those where substituted cysteines were unreactive with any of the probes employed in this study. Residues colored in red and pink represent sites where thiol reactivity was detected toward both channel-permeant and channel-impermeant, thiol-directed reagents. The pink residues are sites where the resulting functional effects were charge-independent, whereas those in red represent sites where functional effects were distinctly charge-dependent. Residues colored in yellow and orange represent those sites where cysteines reacted exclusively with permeant reagents, [Ag(CN)2]− and [Au(CN)2]−. [Ag(CN)2]− reacted at all of these sites while [Au(CN)2]− reacted only at those labeled in orange. All molecular representations were generated using “Visual Molecular Dynamics” (vmd) (54). Image published in: Alexander C et al. (2009) Image downloaded from an Open Access article in PubMed Central. Copyright © 2009 American Chemical Society Larger Image Printer Friendly View

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