Xenbase Image ID: 124441

Figure 2. Tyrosine phosphorylation of cortactin at pre-patterned and nerve-induced AChR clusters.Xenopus muscle cells were labeled with R-BTX and after fixation with an antibody that specifically recognizes Y482-phospho-cortactin (plus FITC-linked anti-rabbit antibodies) (A-F). In some cases muscle cells were first co-cultured for 1 d with spinal neurons and then labeled with R-BTX and anti-phospho-cortactin and secondary antibodies (G-I). In pure muscle cultures (A, D) large “pre-patterned” AChR clusters were present (B, E; arrows) and at these sites staining by anti-phospho-cortactin was significantly stronger than elsewhere in muscle cells (C, F; arrows). Labeling for phospho-cortactin was detected at almost all pre-patterned clusters examined (see Table S1), although within the clusters certain regions at times appeared to be more enriched in phospho-cortactin than others (as in F; arrow versus arrowhead). The anti-phospho-cortactin antibody also labeled muscle cell edges (C, F) where cortactin is known to be localized. In nerve-muscle co-cultures (G) AChRs were selectively concentrated at synaptic contacts (H; arrows) and these nerve-induced AChR clusters were also labeled by the anti-phospho-cortactin antibody (I; arrows). Image published in: Madhavan R et al. (2009) Madhavan et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page