Xenbase Image ID: 124442

Figure 3. Cortactin phosphorylation at AChR clusters induced by growth factor-coated beads and agrin.R-BTX-labeled Xenopus muscle cells were stimulated overnight with HB-GAM-beads (A-C) or neural agrin (D-F). In cells exposed to beads (A; asterisks) AChRs aggregated at bead-muscle contacts (B; arrows) and strong labeling was detected at these bead-induced AChR clusters for Y482-phospho-cortactin (C; arrows). Treatment of muscle cells with agrin (D) generated numerous small (∼0.5-3 µm) AChR clusters (D; arrows) and antibody labeling showed that phospho-cortactin was enriched at these clusters (E; arrows) and also along myopodia that formed near the AChR clusters (F; arrows and arrowheads). Image published in: Madhavan R et al. (2009) Madhavan et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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