Xenbase Image ID: 124584

Figure 2. Voltage-dependent CNGA1 block by extracellular PhTx. (A) Structures of PhTx and spermine (SPM). (B) Macroscopic current traces recorded from inside-out patches containing CNGA1 channels in the presence of the indicated concentrations of extracellular PhTx (in the pipette solution). Currents were activated with 2 mM of intracellular cGMP and elicited with the voltage protocol shown. Dotted lines indicate 0 current levels. (C) Mean I-V curves (mean ± SEM; n = 3–11) determined at the end of the test pulses in the absence or presence of three concentrations of extracellular PhTx. (D) Fraction of current not blocked (mean ± SEM; n = 4–6) by extracellular PhTx is plotted against membrane voltage. Curves are fits of a Boltzmann function (Eq. 1) to the three datasets simultaneously with parameters: appKd (0 mV) = 2.71 ± 0.20 × 10−5 M and Z = 1.76 ± 0.02. Image published in: Martínez-François JR and Lu Z (2010) © 2010 Martínez-François and Lu. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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