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Figure 4. huMgc81475 depletion perturbs spindle assembly.A. Immunofluorescence on fixed HeLa cells after transfection with control and huMgc81475 siRNAs. Cells were stained with anti-phospho-histone H3 (H3P) and anti-tubulin antibodies. Bottom panel shows three representative phenotypes in cells treated with huMgc81475 siRNAs #3 and 4. Size bar – 10 µm. B. Quantification of phenotypes observed as a result of the huMgc81475 knockdown. Morphological stages of mitosis were evaluated for control (white bars; N = 135) and huMgc81475 siRNA-treated H3P-positive cells (black bars; N = 254). C. Percent of mitotic cells with unaligned chromosomes in control- and huMgc81475 siRNA-treated cells. Only mitotic cells with bipolar structures were evaluated. Image published in: Gache V et al. (2010) Gache et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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