XB-IMG-124732
Xenbase Image ID: 124732
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Figure 4. PAGFP diffusion in IS subcompartments. (A) The cell illustrated in Fig. 3 with fluorescence analayzed
in the IS subcompartments indicated by red polygons. The red dot shows
the site of PAGFP activation. (B) Selected images from the time series
in Fig. 3 shown at a higher
frequency and enlarged to reveal the dynamics of photoconverted PAGFP in
the IS region. The color bar is relevant to images in B only. (C)
Integrated fluorescence in each of the regions defined in A, normalized
to the integrated fluorescence of the respective regions in the prepulse
image. Dashed line indicates the expected equilibrium level (see Fig. 3), which will only be reached
when the slowly equilibrating OS is finally at equilibrium. (A and B)
Bar, 10 µm. Image published in: Calvert PD et al. (2010) © 2010 Calvert et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |