XB-IMG-124735
Xenbase Image ID: 124735
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Figure 7. Equilibration of PAGFP in the OS compartment is highly anisotropic. (A)
x–y image of the region of retinal slice
showing the central z level of the cell at which the
experiment was performed. The region of the OS that was rapidly scanned
before and after a 100-µs photoconversion pulse (indicated by the
red symbol) is delineated by the green box. (B) Prepulse scan of the
region showing subregions where time courses of fluorescence change were
recorded (red boxes). (C) Selected time course images showing the rapid
radial and slower axial equilibration. (D) Time courses of fluorescence
changes recorded from the regions shown in B. Radial positions 2 and 3
changed approximately in parallel and merge with the fluorescence time
course from region 1, the site of photoconversion, within ∼2 s.
The fluorescence at axial positions 4 and 5 required much longer,
>15 s, to merge with the fluorescence of region 1, demonstrating
the high degree of anisotropy in PAGFP diffusion in the OS. See Video
3. Image published in: Calvert PD et al. (2010) © 2010 Calvert et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |