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Figure 10. Estimation of the axial diffusion coefficient of PAGFP in the OS compartment. (A) The spatiotemporal profile of activated PAGFP filling of the OS was obtained from the region bounded by the red box. (B) The Dirichlet BC used in calculations was defined by fitting F′DB(t) for n = 5 pixels in z (filled circles) with a sixth-order polynomial (red line). (C) The spatiotemporal profile of activated PAGFP. (D) Eqs. 13–15 were solved with varying D to find the best approximation of the spatiotemporal profile in C. Shown is the model profile, F′m(z,t), obtained from the solution of best fit. The thick black lines in C and D represent the Dirichlet boundary. (E) The data profile was subtracted from the model profile obtained for each value of DOS to determine the difference error. Shown is the difference for D = 0.075 µm2 s−1. (F) RMS error values plotted as a function of D.

Image published in: Calvert PD et al. (2010)

© 2010 Calvert et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license

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