XB-IMG-124739
Xenbase Image ID: 124739
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Figure 11. Estimation of the axial diffusion coefficient of PAGFP within the CC
using the 1-D model. (A) Region over which the fluorescence was averaged
in the radial dimension from each time course image to produce the
spatiotemporal fluorescence profile shown in C. Note that in this case
the region begins at an axial position just proximal to the CC,
z(cc). (B) To produce the Dirichlet BC the proximal
five pixels in the region were averaged and fitted as described in Fig. 10. (C) Spatiotemporal map of
fluorescence changes along the axial extent of the region shown in A.
(D) Model prediction
F′m(z,t)
that best fitted the data. Eqs.
13–15
were solved with spatially varying
D(z):
D(z = IS), 5.2
µm2 s−1;
D(z = OS), 0.08
µm2 s−1;
D(z = CC) was varied to
obtain the best fit of the model to the data. The thick black lines in C
and D represent the Dirichlet boundary constraint. (E) Area of cross
section versus axial distance profile,
A(z), for the region of the cell
analyzed and which was used in Eq. 13 to calculate model profiles; note the sharp drop in
A at the CC. The red symbols denote the transitions
between IS and CC (z(CCl);
left symbol) and the CC and OS
(z(CCL); right
symbol), and define the ranges over which
D(z) values described in D were
applied. (F) RMS error values plotted as a function of
DCC. Image published in: Calvert PD et al. (2010) © 2010 Calvert et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |