XB-IMG-124847
Xenbase Image ID: 124847
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Figure 4. Ago2 expression enhances RNAi in neurons of living Xenopus tadpoles. Neurons, transfected with pSi and pRe constructs, were imaged after 3 or 5 days to evaluate GFP knockdown by G/R fluorescence intensity ratio. (A) Diagram of the pSi (left) containing EnU6-shGFPs with or without Ago protein co-expression, and the pRe (right) for simultaneous GFP and mCherry expression. (B) Representative images from CNS neurons 3 days after transfection of the pSi constructs as indicated. (C) G/R ratios measured from CNS neurons transfected with different pSi constructs driving shGFPs, normalized to control cells. Note that the pSi construct containing the EnU6-shGFPs and Ago2 (red bars) has greater knockdown than the EnU6 promoter alone (green bars) or EnU6-shGFPs with Ago1 (red bars). Asterisks represent statistical significance between neurons transfected with no shRNA and shGFPs with the same silencer configuration (p < 0.05, ANOVA-Scheffe's test). Image published in: Chen CM et al. (2009) Image downloaded from an Open Access article in PubMed Central. Copyright © 2009 Chen, Chiu, Shen and Cline. Larger Image Printer Friendly View |