Xenbase Image ID: 124936

Figure 4. Effects resulting from inhibition of GEMC1 expression in Xenopus embryosa) WB of xGEMC1 extracted from Xenopus embryos that were untreated (Un) or injected with control (C-MO) and morpholino oligos complementary to xGEMC1 DNA sequence (xGEMC1-MO) taken at the indicated stages. Lower panel shows coumassie staining of histone proteins from total embryo lysates. b) Morphology of Xenopus embryos that were untreated (Un), injected with control (C-MO) or anti xGEMC1 morpholino oligos (xGEMC1-MO) taken at the indicated stages. c) Quantification of experiment shown in (b). 100 embryos were counted for each treatment. Graph shows a typical result. d) Sections of fixed Xenopus embryos injected with control (C-MO) or with anti xGEMC1 morpholino oligos (xGEMC1-MO) and stained with ematossilin-eosin. Area in the rectangle shows 5x magnification. e) DNA content reduction in embryos that were uninjected (Not injected), injected with control morpholino oligos (Control MO) or with anti xGEMC1 morpholino oligos (xGEMC1-MO). Data are mean ± SD of 3 independent experiments; * and ** P <0.001 compared with not injected control, t-test) f) Cleavage of 35S-labeled PARP induced by lysates of embryos that were uninjected (Not injected), injected with control morpholino oligos (Control MO) or anti xGEMC1 morpholino oligos (xGEMC1-MO). See Supplementary Fig 6 for un-cropped gels shown in this figure. Image published in: Balestrini A et al. (2010) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

Return to previous page