Xenbase Image ID: 125037

Figure 2. Removal of Nup188–Nup93 enlarges nuclei. (A and C) Western blot analysis of mock- and Nup205–Nup93 (A)- or Nup188–Nup93 (C)-depleted extracts. (B and D) Nuclei were assembled in mock- and Nup205–Nup93 (B)- or Nup188–Nup93 (D)-depleted extracts for 90 min, fixed with 4% PFA and 0.5% glutaraldehyde, and analyzed for chromatin and membrane staining (blue, DAPI; red, DiIC18). (E) Quantitation of the cross-sectional area of mock- and Nup188–Nup93-depleted nuclei of experiments performed as in D. More than 100 randomly chosen chromatin substrates were counted per reaction. The mean of three independent experiments is shown, and error bars represent the total variation. Bars, 20 µm. Image published in: Theerthagiri G et al. (2010) © 2010 Theerthagiri et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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