Xenbase Image ID: 125038

Figure 3. Nup188–Nup93-depleted nuclei contain NPCs and INM proteins. (A) Nuclei were assembled in mock- or Nup188–Nup93-depleted extracts for 90 min, fixed with 4% PFA, and analyzed with Nup188-specific antibodies (green) or the monoclonal antibody mAb414, which recognizes FG-repeat nucleoporins (red). Chromatin is stained with DAPI. (B and C) Samples were prepared as in A and analyzed by immunofluorescence with the indicated antibodies. Bars, 20 µm. Image published in: Theerthagiri G et al. (2010) © 2010 Theerthagiri et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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