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Figure 7. The nuclear growth phenotype and the faster INM targeting are independent of the increased NPC number upon Nup188–Nup93 depletion. (A) Two possible scenarios for the relationship of the nuclear membrane expansion and the increase in NPC numbers upon Nup188–Nup93 depletion. In the top route, Nup188–Nup93 depletion causes a faster growth of the NE, which in turn allows more NPCs (red) to be integrated. In the bottom route, NPC assembly increases on Nup188–Nup93-depleted nuclei. The resulting higher total transport capacity of these allows accelerated nuclear volume and envelope expansion. (B) Nuclei were assembled in mock- and Nup188–Nup93-depleted extracts for 50 min and 120 min. Where indicated, de novo NPC assembly was blocked by the addition of 2 µM importin β after 50 min, i.e., after a closed NE had formed, and nuclei were further incubated for 70 min. NPC numbers per nucleus (green bars) and NPC density (red bars) were quantified. (C) Nuclei were assembled in mock- and Nup188–Nup93-depleted extracts for 50 min. Then, where indicated, de novo NPC assembly was blocked by the addition of 2 µM importin β. INM targeting of the reporter was analyzed as in Fig. 6. Squares are from Nup188–Nup93-depleted samples, and circles from mock-treated samples. Lines mark the mean of two independent experiments. Image published in: Theerthagiri G et al. (2010) © 2010 Theerthagiri et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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