Xenbase Image ID: 125152

Figure 6. Stress-induced increases in CN-Aα levels enhance phosphorylation of PERK and elF2α.(A) Western blot analysis of CN-Aα levels before and after 60 minutes of OGD treatment. Astrocyte cytosolic extracts were resolved on a 12% SDS-PAGE, transferred to nitrocellulose and probed with anti CN-A antibody (Assay Designs cat# SPA-610). A densitometry histogram normalized with actin levels is presented below (n = 5, p<0.05). (B) Co-IP between CN-Aα and PERK corresponding to untreated cells (0 minutes) and OGD treated (30 minutes). The samples were resolved on a 7% SDS-PAGE by loading the CN-Aα immunoprecipitate from astrocytes and transferred to nitrocellulose. The IP was performed with the same anti CN-A antibody, followed by a Western blot with anti PERK antibody (ABGENT cat# AP8054b). A sample from the immunoprecipitate was stained with Coomassie as loading control. Densitometry histogram normalized with Commassie (n = 4, p<0.05). (C) Western blot analysis of CN-Aα levels in astrocytes transfected with siRNA or reagents only (mock) and subsequently treated with vehicle (Veh) or thapsigarin (Tg) for 1 hour. Densitometry histogram is normalized with actin (n = 4, p<0.01). (D) Western blots of astrocyte extracts probed with anti P-eIF2α antibody. Densitometry histogram is normalized with actin (n = 4, p<0.01). Image published in: Bollo M et al. (2010) Bollo et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page