Xenbase Image ID: 125153

Figure 7. Knockdown of CN-A increases the appearance of apoptotic nuclei in Xenopus oocyte extracts.(A) Apoptotic potency of cytosolic extracts obtained from control oocytes treated with Tg (Cntrl + Tg) or from oocytes injected with CN-A morpholino 1 & 2 treated with Tg (Morpho CN 1&2 + Tg) compared with buffer alone. Images of liver nuclei were obtained at 2 hours. Note the large number apoptotic-like nuclei at 2 hours for Morpho + Tg oocyte extract. (B) Lineplot of the average of the percentage of apoptotic nuclei at 0, 2 and 4 hours for cytosolic extract obtained from control oocytes with and without Tg treatment (Cntrl, Cntrl + Tg), from oocytes injected with standard morpholino with and without Tg treatment (Std Morpho, Std Morpho + Tg) and from oocytes injected with CN-A morpholino oligos 1 & 2 with and without Tg treatment (Morpho CN1&2, Morpho CN1&2+ Tg), compared to nuclei incubated with buffer alone. Data were obtained from 4 independent experiments in which 150 oocytes per group were used for each condition. **p<0.01. Image published in: Bollo M et al. (2010) Bollo et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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