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Figure 3. Germ plasm RNAs fail to localize to the cortex of mgn mutant oocytes.vasa (A–D) and dazl (E–H) in situ hybridization to wild-type and mgn mutant oocytes. vasa mRNA is found in the Balbiani body in early stage I wild-type (heterozygous) oocytes (A, arrow), transiently localizes to the vegetal cortex in late stage I (A, arrowhead) and then localizes all around the cortex during stage II (B). In mgn mutant oocytes, vasa mRNA is found in the Balbiani body in early stage I oocytes (C, arrow) and is then found ectopically localized to the middle of the oocyte during stage II (D, arrow). In early stage I wild-type oocytes, dazl mRNA is found in the Balbiani body (E, arrow) and then localizes to the vegetal cortex during late stage I (E, arrowhead) and stage II (F). dazl mRNA localizes to the Balbiani body of early stage I mgn mutant oocytes (G, left oocyte, arrow) and is found ectopically localized to the middle of the oocyte during late stage I and stage II (G, right oocyte and H, respectively, arrow). Scale bars = 50 microns. Image published in: Gupta T et al. (2010) Gupta et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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