Xenbase Image ID: 125301

Figure 9. Effect of 1 mM MgADP on Kir6.2-G334D/SUR1 kinetics. (A and C) Single Kir6.2-G334D/SUR1 currents recorded at −60 mV from the same patch first in nucleotide-free solution (A), and then in 1 mM MgADP (C). (B and D) Distributions of open times (left), closed times (middle), and burst durations (right) in 0 mM MgADP (B; PO = 0.39) and 1 mM MgADP (D; PO = 0.82). The distributions were fit with the following parameters. For 0 mM MgADP (B): open times: τO = 2.31 ms (τO,COR = 1.51 ms); closed times: τC1 = 0.33 ms, aC1 = 0.942; τC2 = 7.3 ms, aC2 = 0.021; τC3 = 36 ms, aC3 = 0.034; τC4 = 230 ms, aC4 = 0.003; burst times: τB1 = 41 ms, aB1 = 0.770; τB2 = 5.6 ms, aB2 = 0.115; τB3 = 0.46 ms, aB3 = 0.115. The mean burst duration (τ) was 32.3 ms. For 1 mM MgADP (D): open times: τO = 2.61 ms (τO,COR = 1.77 ms); closed times: τC1 = 0.38 ms, aC1 = 0.990; τC2 = 3.8 ms, aC2 = 0.001; τC3 = 14 ms, aC3 = 0.009; burst times: τB ≡ τB1 = 263 ms. Image published in: Proks P et al. (2010) © 2010 Proks et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page