XB-IMG-125327
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Figure 2. N-terminal regions of DAAM1 are involved with actin stress fibers and centrosome targeting.(A) GFP-Flag-DAAM1 (1–440) was expressed in HeLa and analyzed by confocal imaging. DAAM1 was immuno-localized with anti-GFP (in green). Cells were counter-stained with TRITC-phalloidin, anti-myosin IIA or anti-myosin IIB (red). (B) Table summarizing the localization of various DAAM1 constructs tested in this study. (C) COS-7 cells expressing DAAM1(100–350) form large filaments enriched for myosin IIB that do not colocalize with F-actin. Treatment with various acto-myosin disrupting drugs with the indicated concentration for 45 min do not abolish the presence of these fibrils. Bars = 10 µm. Image published in: Ang SF et al. (2010) Ang et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |